- Computer-Aided Drug Design (CADD)
- DNA-Encoded Library Technology (DELT)
- Fragment-Based Screening
- High Content Screening (HCS)
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High Throughput Screening (HTS)
- Automated HTS Platform
- Biochemical assays in Hit Characterization
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Biophysical Assays in Hit Characterization
- BLI for Affinity-based Hit Screening
- CD Spectrometry for Protein Structure Determination
- ITC for Binding Assessment
- MS for Structure Confirmation
- MT for Binding Affinity Measurement
- NMR Spectrometry for Tareget identification and Characterization
- SPR Spectrometrys for Structure Determination
- TSA for Protein's Stability Evaluation
- Cellular assays in Hit Characterization
- Drug Repurposing
- Hit Screening
- HTS Assay Development
- HTS Compounds Libraries
- HTS Data Management
- Virtual Screening (VS)
One-stop
Drug Discovery Services
- Experienced and qualified scientists functioning as project managers or study director
- Independent quality unit assuring regulatory compliance
- Methods validated per ICH GLP/GMP guidelines
- Rigorous sample tracking and handling procedures to prevent mistakes
- Controlled laboratory environment to prevent a whole new level of success
High Content Analysis
INQUIRYHigh content analysis is a studying disease-relevant assay and applied for optimize the hits from high content screening. Once a hit has been identified, you can use the high content analysis to progress the candidate through lead optimization with the reliable data delivered by HCA. As a new generation of cell-based assay, high content analysis enables to predict the efficacy and toxicity of therapeutic drugs in vivo more accurately and give critic indication before moving into a safety assessment.
Fig.1 High content imaging and analysis (A) Schematic of the assay design. (B) Schematic of a 96-well optical plate which is typically using in high content imaging system. (C) Schematic of High content Analysis. (Lee, H. W.; et al. 2015)Services of High Content Analysis
- We provide multiplexing high throughput technologies to analyze multicellular structures such as 2D cell cultures, 3D spheroids and co-cultures.
- Ou teams can monitor various features within a microenvironment all in one well and extract quantitative and valuable data from cell populations.
- We perform a set of detailed assays for apoptosis including: Cytotoxicity, cell migration, cell proliferation and neurite outgrowth,etc.
- With professional knowledge in biology, our scientists are capable of evaluating the biological impact of compounds in conditions that preserve crosstalk and feedback mechanisms relevant to in vivo outcomes.
- We can also provide important information about potency, selectivity, safety, side effects, mechanism of action, and disease indication of your compound, delivering the right molecule candidate.
- Our HCA experts have designed numerous complex assay algorithms and applied them as important analysis tools in rapid batch processing during large-scale high content screening.
- We format and organize the generated data for ranking, clustering, statistics, and machine learning, providing reliable predictive analytic for your hit identification.
Our Advantages of High Content Analysis Services
- Our HCA platforms are equipped with state-of-the-art machines with the deep learning function for complex sample analysis and support the analysis of various phenotypic data and target reaction.
- We offer numerous novel technologies including better image analysis algorithms, artificial intelligence, more physiologically relevant cell types and cell cultures, etc., aiming to give guidance on your drug discovery assays.
Equipment
BOC Sciences has developed a powerful integrated approach to perform and automate high-content analysis:
HCS platform: Thermo Scientific CellInsight CX7 LZR
Reference
- Lee, H. W.; et al. A Podocyte-Based Automated Screening Assay Identifies Protective Small Molecules. Journal of the American Society of Nephrology: JASN. 2015, 26: 2741.
※ It should be noted that our service is only used for research.
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