Computer-Aided Drug Design
- ADME/Tox Prediction
- De Novo Drug Design
- Ligand-Based Virtual Screening
- Quantum Mechanics
- Structure-Based Virtual Screening
- DNA-Encoded Library Technology
- Fragment-Based Screening
- High Content Screening
High Throughput Screening
- Assay Development
- Automated HTS Platform
- Biochemical Assays
- Bio-Layer Interferometry
- Circular Dichroism Spectroscopy
- Isothermal Titration Calorimetry
- Mass Spectrometry
- Microscale Thermophoresis
- Nuclear Magnetic Resonance Spectrometry
- Surface Plasmon Resonance Spectrometry
- Thermal Shift Assay
- Cellular Assays
- Compound Libraries
- Data Management
- Drug Repurposing
- Hit Screening
- Virtual Screening
- Experienced and qualified scientists functioning as project managers or study director
- Independent quality unit assuring regulatory compliance
- Methods validated per ICH GLP/GMP guidelines
- Rigorous sample tracking and handling procedures to prevent mistakes
- Controlled laboratory environment to prevent a whole new level of success
What is Hit Screening?
The main objective of high throughput screening is to select the hits that have desired effect on target of our interest. The general route of hit screening is firstly identifying hits with required inhibition/activation effects through compound libraries screening, and then validating these activities with the use of diverse analytic methods.
Fig.1 The picture of hit screening
·High throughput screening of libraries of molecules
BOC Sciences has a diverse collection of more than 30,000 compounds for our scientist to perform full-scale hit identification assays. Our screening libraries allow target- or ligand-based pre-screening using in silico algorithms. Our fragment libraries that containing more than 20 000 molecules are also available for fragment-based screening. We also offer other diverse sub-libraries to improve the success rate in tracking hit molecules.
Statistical tests are applied to identify hits and we conduct screening with replicates. Our experts use the data obtained to calculate the variability and use the t-statistic to assess the significance and highlight hits.
Once a hit is selected, our teams carry out further verification testing with the application of a variety of methods in the hit confirmation phase of your drug discovery projects.
Enzymatic assays (active enzymes such as deacetylases, acetyl-transferases, kinases, phosphatases, etc.).
Protein-protein interaction study (involving peptide-protein interaction and other larger macromolecular complexes).
2.Cell based HTS
Phenotype screening and imaging based on primary cells.
Compound profiles such as, cytotoxicity data, biological annotations of hit compounds and kinase selectivity profiles.
Structural characterization of hit compound clusters.
BOC Sciences has abilities in identifying hits from a large amount of compounds with our diverse compound libraries and experience in running large-scale high-throughput assays.
We provide multiple pre-screen orthogonal assay technologies with valid readout to increase the probability of success of your drug discovery program.
※ It should be noted that our service is only used for research.