LC-MS for Target Separation and Detection

LC-MS for Target Separation and Detection

Services

One-stop

Drug Discovery Services

  • Experienced and qualified scientists functioning as project managers or study director
  • Independent quality unit assuring regulatory compliance
  • Methods validated per ICH GLP/GMP guidelines
  • Rigorous sample tracking and handling procedures to prevent mistakes
  • Controlled laboratory environment to prevent a whole new level of success

LC-MS for Target Separation and Detection

INQUIRY

Liquid Chromatography-Mass Spectrometry (LC-MS)

Chromatogram and Mass Spectrum.

Fig.1 Chromatogram and Mass Spectrum. (Sarvani, V.; et al. 2013)

Introduction

Liquid chromatography-mass spectrometry (LC-MS) has developed to become a powerful, indisposable and essential bioanalytical technology. A high-throughput LC-MS process require conditions for separation and detection of excellent reproducibility, robustness, and low fault liability. Early drug discovery projects make full use of LC-MS screening approaches which selectively detect chemical functionalities of target molecules. Liquid chromatography conduct safe separation of a wide range of organic compounds from small-molecule drug metabolites to peptides and proteins. Chromatographic process is therefore applied as a separation technique in mass spectrometry helping in both qualitative and quantitative analysis of candidate drugs.

Advantages

LC-MS has several advantages over MS and they can be summarized as the following aspects:

  • A large numbers of drugs can be identified in just one condensed analysis window.

  • As a presumptive method, LC-MS greatly reduces the use of reagent which is cost-effective for the drug discovery project.

  • LC-MS can rapidly generate better data quality and is easy to operate.

Process

The whole process of LC-MS can involve the following three steps:

  • Separate mixtures in accordance with their physical and chemical properties.

  • Identify the components within each peak.

  • Detect each of them on the basis of their mass spectrum.

Services

We have adapted LC-MS in different stages of drug discovery and provided multiply services to meet your demand.

  • Determination of the molecular weight with the support of LC-MS for analysis of lead candidate.

  • Protein identification and metabolite stability profile using LC-MS in the lead candidate validation.

  • Confirmation of peptide and glycoprotein identification in target identification.

  • Bio-affinity screening analysis is performed with LC-MS in lead identification.

  • Pharmacokinetic screening and metabolic stability analysis in the lead optimization.

Equipment

Agilent 6550 iFunnel Q-TOF LC-MS

Reference

  • Sarvani, V.; et al. Role of LC-MS in drug dicovery process. International Journal of Pharmacy & Therapeutics. 2013, 4(3): 148-153.

※ It should be noted that our service is only used for research.