DNA-Encoded Library Design and Synthesis

DNA-Encoded Library Design and Synthesis

Services

One-stop

Drug Discovery Services

  • Experienced and qualified scientists functioning as project managers or study director
  • Independent quality unit assuring regulatory compliance
  • Methods validated per ICH GLP/GMP guidelines
  • Rigorous sample tracking and handling procedures to prevent mistakes
  • Controlled laboratory environment to prevent a whole new level of success

DNA-Encoded Library Design and Synthesis

INQUIRY

DNA-encoded chemical libraries are hybrid-type collections of diverse organic molecules and each of them is coupled to distinctive DNA fragments. These unique libraries are generated by iterative combinatorial synthesis and each molecule is linked to a distinct DNA tag which records its synthetic process. The designed libraries consist various novel structures that cover a wide chemical space including diversified scaffolds, which are suitable for further hit-to-lead optimization.

Fig.1 Schematic representation of virtual screening approaches. (Polgar, T.; Keseru, G. M. 2011)Fig.1 Schematic of DNA-encoded libraries synthesis. (Verena, K.; et al. 2018)

Design of DELs

Our chemists consider both chemistry-driven and biology-driven methods to design libraries:

  • Chemistry-driven library design

1.We carry out this type of libraries design by employing DNA-compatible chemical reactions and synthesis strategies.

2.Many novel and drug-like scaffolds as well as building blocks are also used in the design of DELs.

  • Biology-driven library design

1.We design libraries based on structural information of target types with specific biological effect to cover and expand chemical spaces of drugs or bioactive molecules.

Our Synthesis Process

BOC Sciences has developed a complete set of DELs production processes which ensure the high efficiency and quality of DNA-encoded libraries.The detailed production process is as follows:

We apply the 'split & pool' synthesis strategy to build our DNA-encoded libraries. In this approach, a building block (BB) is tagged with a DNA sequence and then a second one is added subsequently. The DNA tag is able to be lengthened with a sequence that corresponds to the next BB. At each step, BBs are added to mixtures of thousands of different tagged BBs and the libraries can grow to vast sizes quickly.

Our Other Services

  • Quality control

Aiming to monitor ligation reaction, multiple quality control approaches are applied during the process of DEL production including UPLCMS, HPLC and electrophoresis.

  • Automation of DEL synthesis

At BOC Sciences, we have established an automatic synthesis platform to perform the synthesis of DELs.

Our Advantages

  1. All building blocks are validated and analyzed under certain conditions to get the assessment of yields and by-product, and only building blocks which meet the standard criteria will be selected for our DEL production.

  2. Various quality control methods developed in-house, such as electrophoresis and quantification (by Qubit, OD, bioanalyzer and qPCR) are available to evaluate the quality of the final product.

  3. Our advanced automatic synthesis platform can improve the efficiency and homogeneity of parallel reactions and 'split & pool' operations, which ensures the the library quality.

  4. With years of accumulation of chemical knowledge, BOC Sciences has established a DNA-encoded compound library with structural diversity and high quality based on the technologies of diverse molecular building blocks.

  5. We leverage our unique chemistry knowledge and apply robust reactions to develop and deliver novel, customized DELs for our clients.

Reference

  • Verena, K.; et al. DNA-encoded libraries - an efficient small molecule discovery technology for the biomedical sciences. Biological Chemistry. 2018, 399(7).

※ It should be noted that our service is only used for research.

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