Off-DNA Compound Re-synthesis

Off-DNA Compound Re-synthesis

Services

One-stop

Drug Discovery Services

  • Experienced and qualified scientists functioning as project managers or study director
  • Independent quality unit assuring regulatory compliance
  • Methods validated per ICH GLP/GMP guidelines
  • Rigorous sample tracking and handling procedures to prevent mistakes
  • Controlled laboratory environment to prevent a whole new level of success

Off-DNA Compound Re-synthesis

INQUIRY

After analyzing the DEL selection data, hit compounds (on-DNA) are typically re-synthesized without the DNA tag. Generally, enriched hits can be re-synthesized as fluorescent conjugates to enable fluorescence-based assay formats. Once the re-synthesis of these 'off-DNA' compounds is completed, followed by the confirmation process.

Fig.1 Schematic of on-DNA screening and off-DNA screening. (Hackler, A. L.; et al. 2015)Fig.1 Schematic of on-DNA screening and off-DNA screening. (Hackler, A. L.; et al. 2015)

Our Process

  • Generate multi-mapped reads for each sample.

  • Enrich signals.

  • Design different scenario for feature analysis.

  • Hit proposal.

  • Re-synthesize the most enriched compounds 'off-DNA'.

Our Capacities

We have the following considerations in the whole hit proposal process:

  • Signal intensity (sequence count, enrichment feature intensity).

  • Chemical type diversity.

  • Chemical and physchem properties of compounds.

  • Structural associations and mechanism of action between different DNA-encoded compound libraries (signal comparison between samples)

  • We can re-synthesize hit compounds with a fluorophore moiety or other chemical moieties.

  • For the re-synthesis, 5 mg is offered for each compound and for up to 100 compounds, with less than 8 weeks lead time.

Our Advantages

  1. We have a professional and experienced chemical team and our experts have participated in hundreds of re-synthesis projects.

  2. BOC Sciences is equipped with advanced equipment and instruments. Moreover, a diversity of reagents in the libraries are available for best synthesis conduction.

  3. We can also offer gram-level synthesis for active compounds, compound skeletons, etc.

Reference

  • Hackler, A. L.; et al. Off-DNA DNA-Encoded Library Affinity Screening. ACS Combinatorial Science. 2019, 22(1): 25-34.

※ It should be noted that our service is only used for research.

Online Inquiry

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